An unskilled worker in a water garden was sent to sweep up a spill of white powder in the storage shed. Later, he was found with labored breathing and convulsions. On further examination, the white powder was identified as rotenone. Respiratory distress is induced by rotenone exposure because it inhibits the complex that catalyzes which […]
Electron transport chain inhibition by Rotenone Read More »
As electrons are received and passed down the transport chain, the electron carriers are first reduced with the acceptance of the electron and then oxidized with loss of the electron. A patient poisoned by which of the following compounds has the most highly reduced state of most of the respiratory chain carriers? A. Antimycin A
Reduced state of the respiratory chain carriers (Inhibition of electron transport chain) Read More »
Electrophoresis is the movement of charged particles through an electrolyte when subjected to an electric Field- Cations move towards the cathode Anions move towards the anode By this technique, solutes are separated by their different rates of travel through an electric field. Commonly used in biological analysis, particularly in the separations of proteins, peptides, and nucleic acids Factors affecting
Electrophoresis – A brief review Read More »
Chromatography from Namrata Chhabra
Chromatography-an overview Read More »
ELISA– Enzyme-Linked Immunosorbent Assay The ELISA techniques are widely used for- 1) Quantitative estimation of- Hormones Amino acids Growth factors Tumor markers and Other analytes which are present in a very small concentration in biological fluids 2) Qualitative detection of- Bacterial or viral antigens Antibodies against microbes Any antigen, or antibody present in a small
ELISA- A quick review Read More »
The word Chromatography is derived from two Greek words –Chroma means – color and graphein to write. Chromatography is the collective term for a family of laboratory techniques for the separation of mixtures. Chromatography, literally “color writing”, was used—and named— in the first decade of the 20th century, primarily for the separation of plant pigments
Introduction A colorimeter is a device used in colorimetry. The word generally refers to the device that measures the absorbance of particular wavelengths of light by a specific solution. This device is most commonly used to determine the concentration of a known solute in a given solution by the application of the Beer-Lambert law. Principle
COLORIMETRY- Simplified Read More »
Q.1- Choose the incorrect statement out of the following for the human immune deficiency virus a) The reverse transcriptase enzyme is a characteristic feature of retroviruses b) p10 is a protease that cleaves gag precursor c) p32is an Integrase d) gag encodes for the lipid bilayer of the virus. Q.2- The specific binding of HIV to the CD
HIV Infection-Multiple-choice questions Read More »
Case details A- 29-year-old officer presented to the emergency department complaining of chills and breathing difficulty. He was a heroin abuser who was admitted to the same hospital 7 years ago because of drug overdosage. He had lost 10 kg of body weight since his last clinical visit. On examination, he had multiple enlarged lymph
Case study- HIV Infection Read More »
